Effects of 2-phenoxyethanol on survival of normal juveniles and malformed juveniles having lordosis or nonfunctional swimbladders of European sea bass (Dicentrarchus labrax L., 1758)


BAŞARAN F., Sen H. , Karabulut S.

AQUACULTURE RESEARCH, cilt.38, ss.933-939, 2007

Özet

The objectives of this study were to evaluate the effects of 2-phenoxyethanol (2-PE), which is an anaesthetic, on survival rates of normal juveniles and malformed juveniles having lordosis or nonfunctional swim bladders of European sea bass (Dicentrarchus labrax L., 1758) and to establish the LC50 (the concentration lethal to 50% of test animals at concentrations of 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4 and 4.5 mL L-1) and LT50 (the time lethal to 50% of test animals after 10-, 20-, 30-, 40-, 50- and 60-min time periods) of 2-PE at 19 +/- 0.5 degrees C, salinity 38 g L-1, pH 7.4-7.8 and dissolved oxygen > 8 mg L-1. Between concentrations of 0.05 and 0.25 mL L-1, 2-PE did not cause any mortality or toxicity on normal, lordosis and nonfunctional swimbladder juveniles of sea bass during the 60-min exposure period. On the other hand, significance in each group fish in their mortality rates between concentrations of 0.30 and 0.45 mL L-1 was observed (P < 0.05). The nonfunctional swimbladder juveniles showed lower LC50 than normal and lordosis juveniles respectively. Also, nonfunctional swimbladders juveniles showed lower LT50 than normal and lordosis juveniles respectively. At concentrations of 0.30, 0.35, 0.40 and 0.45 mL L-1, induction times were found to be significantly different among the three groups (P < 0.05). Recovery times were not found to be significantly different in two groups at concentrations of 0.30 and 0.40 mL L-1 (P > 0.05). The toxic effect of 2-PE on sea bass juveniles increased depending on the exposure times (P < 0.05). The most suitable concentrations of 2-PE were 0.30-0.35 mL L-1 between minutes 10 and 30, although the normal juveniles can resist to 0.45 mL L-1 of 2-PE concentration for 20 min. The 2-PE showed toxicity in relation to the concentrations and exposure time combinations among the three groups in the order; nonfunctional swimbladder fish > lordosis fish > normal fish.