The Synergistic Effect of Fotemustine and Genistein on Expressions of p53, EGFR and COX-2 Genes in Human Glioblastoma Multiforme Cell Line


Avci C. B. , Dodurga Y., Oktar N., Susluer S. Y. , Sigva Z. O. D. , Gunduz C.

JOURNAL OF NEUROLOGICAL SCIENCES-TURKISH, cilt.29, ss.510-517, 2012 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 29 Konu: 3
  • Basım Tarihi: 2012
  • Dergi Adı: JOURNAL OF NEUROLOGICAL SCIENCES-TURKISH
  • Sayfa Sayısı: ss.510-517

Özet

GBM is the most common primary malignant neoplasm of the central nervous system in adults. Fotemustine (FTM) is a cytotoxic alkylating agent and a lipophilic chloroethylnitrosourea derivative. Its mechanism of action consists mainly in inducing DNA strand breaks and cross-linking. Genistein, one of the soy-derived isoflavones, exerts its anticancer properties via several mechanisms, including inhibition of tyrosine phosphorylation, weak estrogenic and anti-estrogenic properties, as an antioxidant, inhibition of topoisomerase II, inhibition of angiogenesis, and induction of cell differentiation in a number of human tumors. We aimed to investigate the anti-proliferative synergistic effect of genistein with fotemustine on human glioblastoma multiforme U87-MG cells. This study was also designed to answer the following question: Do the p53, EGFR, COX-2 genes' expression patterns differ in treatment of these both drugs alone and in combination?