Morphological and phylogenetic identification of Botrytis cinerea causing blossom blight and fruit rot of sweet cherries in Aegean region, Turkey


Uysal-Morca A., KINAY TEKSÜR P. , Egerci Y.

JOURNAL OF PLANT DISEASES AND PROTECTION, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume:
  • Publication Date: 2021
  • Doi Number: 10.1007/s41348-021-00482-7
  • Title of Journal : JOURNAL OF PLANT DISEASES AND PROTECTION

Abstract

Gray mold disease (Botrytis cinerea Pers.: Fr.) of sweet cherries causes blossom blight and fruit rot during pre- and post-harvest stages. This study was aimed at morphological and molecular identification of the causal agents of gray mold disease of sweet cherry in Turkey. The surveys were conducted in the Aegean region of the country during 2015, 2016 and 2017. The surveys indicated that prevalence of B. cinerea causing blossom blight ranged from 7.05 to 27.01%, while B. cinerea causing fruit rot was observed on 1.15-5.34% trees in the surveyed orchards. In addition, prevalence of B. cinerea causing fruit rot ranged from 1.56 to 6.42% in fruit collection stations and packaging houses. The B. cinerea was identified based on the culture and microscopic characteristics of the collected isolates. All isolates of B. cinerea proved pathogenic in pathogenicity tests. The phylogenetic analysis using the sequences of three nuclear protein-coding genes indicated that glyceraldehyde-3-phosphate dehydrogenase gene, heat shock protein 60 gene, and DNA-dependent RNA polymerase subunit II gene were clustered in the same branch with reference isolates of B. cinerea. The transposable elements of Boty and Flipper were used to determine the genetic differences among the isolates. The Boty was present in all isolates of, while the Flipper was present only in four isolates. The presence of B. cinerea causing blossom blight disease in sweet cherry is reported for the first time from Turkey through this study.