Lactococcus garvieae is one of the main pathogenic agents in rainbow trout farms in Turkey. Twenty-two L. garvieae isolates obtained from different regions in Turkey were evaluated phenotypically in the study. In all isolates, cream colored, bright, round and S-type colonies with smooth margins were seen in TSA medium. They were alive during native examination without movement. It was observed that morphologically all isolates were Gram (+), alpha-hemolytic (BA), oxidase and catalase negative and were reproduced under 21, 37 and 45 degrees C temperatures with 0-6.5 % NaCl salinity. As a result of the examination of biochemical properties with API Rapid ID 32 Strep test, it was observed that 2 L. garvieae isolates were different from other isolates in respect of sucrose utilization. 1 and 19 number isolates were negative for sucrose whereas other isolates gave positive results. 1 number isolate was different from other isolates based on maltose profile. While isolate 22 was maltose negative, the other isolates gave positive results. According to phenotypic differences, all isolates used in the study were classified as three different groups.