Assessment of antimicrobial activity of nanosized Ag doped TiO2 colloids


YAŞA İ. , LKHAGVAJAV N., KOIZHAIGANOVA M., Celik E., SARI O.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.28, no.7, pp.2531-2539, 2012 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 7
  • Publication Date: 2012
  • Doi Number: 10.1007/s11274-012-1061-y
  • Title of Journal : WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Page Numbers: pp.2531-2539
  • Keywords: Ag doped TiO2 colloids, Nanoparticles, Sol-gel, Antimicrobial activity, MIC value, SILVER-COATED TIO2, THIN-FILMS, GLASS SUBSTRATE, PHOTOCATALYTIC ACTIVITY, ANTIBACTERIAL, NANOPARTICLES, PARTICLES, MECHANISM, MEDICINE

Abstract

In the present research, the antimicrobial effects of nanosized silver (Ag) doped TiO2 colloidal solutions prepared using a sol-gel technique were investigated. In order to determine the solution characteristics, the turbidity, viscosity and pH of the colloidal solutions were measured. Differential thermal analysis-thermogravimetry equipment was used to determine the chemical structures and reaction types of the films formed from these solutions. The morphology of Ag doped TiO2 nanoparticles was evaluated by atomic force microscopy. The disc diffusion method was employed to explore antimicrobial activity, and the Broth Microdilution method was used to obtain MIC values of nanosized Ag doped TiO2 colloidal solutions against the test microorganisms Escherichia coli, Staphylococcus aureus, Candida albicans, Bacillus subtilis, and Salmonella typhimurium. It was found that the silver doped TiO2 nanoparticles inhibited the growth and multiplication of the test microorganisms, including the fungus C. albicans. Antimicrobial activity was observed against all tested microorganisms at a very low concentration of 1.125-2.81 mu g/ml of nano silver in 1-25 % Ag-TiO2 solutions.