Impedimetric biosensor for cancer cell detection


SEVEN B., BOUROUROU M., ELOUARZAKİ K., CONSTANT J. F. , GONDRAN C., HOLZİNGER M., ...Daha Fazla

ELECTROCHEMISTRY COMMUNICATIONS, cilt.37, ss.36-39, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 37
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.elecom.2013.10.003
  • Dergi Adı: ELECTROCHEMISTRY COMMUNICATIONS
  • Sayfa Sayıları: ss.36-39

Özet

Electrochemical impedance spectroscopy was evaluated for the label free detection of MCF-7 cancer cell in which c-erbB-2 receptor is overexpressed on the cell surfaces. Anti-c-erbB-2, used as a specific antibody, was immobilized on electrogenerated polypyrrole-NHS on electrodes via covalent linking. The polymer formation, the grafting of the antibody, and the recognition event with the cancer cells using MCF-7 as a model cell line, were characterized by using cyclic voltammetry and fluorescence microscopy. The impedimetric sensor showed high sensitivity from 100 to 10 000 cell/mL without needing any labeling step and represents an efficient transduction method for cell selective detection. (C) 2013 Elsevier B.V. All rights reserved.