Controlled release of anticancer drug Paclitaxel using nano-structured amphiphilic star-hyperbranched block copolymers


GEYİK C. , Ciftci M., DEMIR B., GULER B., OZKAYA A. B. , GÜMÜŞ Z. P. , ...Daha Fazla

POLYMER CHEMISTRY, cilt.6, ss.5470-5477, 2015 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 6 Konu: 30
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1039/c5py00780a
  • Dergi Adı: POLYMER CHEMISTRY
  • Sayfa Sayıları: ss.5470-5477

Özet

In the present study, two amphiphilic star-hyperbranched copolymers based on poly(methyl methacrylate)-b-poly(2-hydroxyethyl methacrylate) (PMMA-b-PHEMA), with different hydrophilic PHEMA segment contents (PMMA-b-PHEMA-1, and PMMA-b-PHEMA-2), were synthesized, and their drug loading and release profiles were examined using Paclitaxel (PTX) as a model drug. The drug loading capacities and encapsulation efficiencies were found to be similar in both polymers. The encapsulation efficiencies were found to be prominent at 98% and 98.5% for PMMA-b-PHEMA-1 and PMMA-b-PHEMA-2, respectively. On the other hand, the drug release behaviors varied in favor of the block copolymer comprising shorter PHEMA chains (PMMA-b-PHEMA-1). Additionally, to assess the biological effects of PTX-loaded polymers, human non-small cell lung carcinoma (A549) cells were used. Cell viability and cell cycle analysis showed that both polymers were non-toxic to cells. The cytotoxic effect of PTX-loaded PMMA-b-PHEMA-1 on A 549 cells was greater (66.49% cell viability at 5.0 ng mL(-1) PTX) than that of PMMA-b-PHEMA-2 (72.47% cell viability at 5.0 ng mL(-1) PTX), consistent with the drug release experiments.