Research Information System
Fresenius Environmental Bulletin, no.29, pp.2691-2697, 2020 (Journal Indexed in SCI)
The
aim of the present study was to establish a protocol for cryopreservation of
sperm in Sparus aurata and
also to verify the applicability under culture conditions. Cryopreservation of
sperm was attempted by using two extenders: Extender A (10 % egg yolk, buffer
solution) and Extender B (1% NaCl, 10% BSA Bovine Serum Albumin). Cryoprotectants
were assessed at three different concentrations (5, 10 and 15 %, DMSO)
individually as well as in combination with varying equilibration times (10 and
30 min). In order to maintain freezing rate, freezing protocols were adjusted
by −30 °C/min intervals. Obtained results indicated that cryomedium
constituting of Extender A with addition of 10% DMSO in dilution ratio of 1:3
(sperm:cryomedium) at an equilibration time of and freezing rate of −30 °C/min
was more desirable compared to the other experimental procedures (Extender B)
that were assessed (P>0.05). Moreover, 40 °C for 10 s presented highest
post-thaw motility after end of the first month (72,7±0,35%) (P>0.05).
Finally, it is determined that Extender A exhibited better performance in terms
of sperm motility, freezing and thawing rate and also this ratio could be
effectively utilized for cryopreservation of sperm for this species.