Chemical Constituents and Antimicrobial Activity of the Leaves of Eucalyptus (Eucalyptus camaldulensis Dehnh.), An Endemic Plant from West Anatolia


Ceyhan N. , Keskin D., Zorlu Z.

JOURNAL OF PURE AND APPLIED MICROBIOLOGY, vol.6, no.3, pp.1447-1453, 2012 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 3
  • Publication Date: 2012
  • Title of Journal : JOURNAL OF PURE AND APPLIED MICROBIOLOGY
  • Page Numbers: pp.1447-1453

Abstract

The extracts of methanol water, hexane and chloroform eucalyptus leaves (Eucalyptus camaldulensis Dehnh.) were screened in vitro for their antimicrobial activity against some pathogenic gram negative, gram positive bacteria and one yeast. Disk diffusion method was used for antimicrobial activity of extracts and the microwell dilution assay was used for the determination of minimal inhibitory concentration (MIC) of each extract. In addition, the composition of chloroform extratc was determined by means of GC/MS analysis. The methanol/water, hexane and chloroform exracts of E.camaldulensis leaf showed various antimicrobial activity 7-9mm, 8-11mm and 11-27 mm inhibition zone, respectively. The chloroform extracts was found to be the most effective antimnicrobial agent as compared to the other extracts. While the most sensitive bacteria was Bacillus cereus CCM 99 (27mm inhibition zone), the most resistant bacteria was Esherichia coli ATCC 35218. The methanol/water, hexane and chloroform extracts of E. camaldulensis leaf showed 1.25-5.0