Arginine selective biosensor based on arginase-urease immobilized in gelatin


KARACAOĞLU S., Timur S. , TELEFONCU A.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.31, ss.357-363, 2003 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 31 Konu: 3
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1081/bio-120023164
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.357-363

Özet

Arginase and urease enzymes were immobilized on the surface of pH electrode by using gelatin membrane which is then cross-linked with glutaraldehyde. Sensor response was maximum when 2.5 mM, Tris-HCl buffer (pH 8.5) was used at 25degreesC. The biosensor response depends linearly on arginine concentration between 0.025-0.310 mM with response time 10 min. Furthermore, application of the system for the arginine detection in serum samples was also tested.