Parametric continuous feed stream design to fine-tune fed-batch bioreactor performance: recombinant human growth hormone production in Bacillus subtilis


Calik G., Kocabas P. , Afsar H., ÇALIK P., Ozdamar T. H.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.91, no.11, pp.2740-2750, 2016 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 91 Issue: 11
  • Publication Date: 2016
  • Doi Number: 10.1002/jctb.4864
  • Title of Journal : JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Page Numbers: pp.2740-2750

Abstract

BACKGROUND: Design principles of a fed-batch bioreactor system to fine-tune bioreactor performance by increasing production and productivity are investigated. To enhance the production and productivity in fed-batch fermentation of recombinant human growth hormone (rhGH) by Bacillus subtilis, this work develops a continuous feed stream (CFS) design method with the parameters, cultivation time to switch batch-to fed-batch operation, so called transition time, t(T), and specific growth rate, mu.