Objective: The purpose of this study was to compare the agar proportion method with the E- test method for susceptibility testing of Mycobacterium tuberculosis. Materials and Methods: A total of 100 isolates were tested for isoniazid, rifampin, streptomycin and ethambutol susceptibility using an indirect- proportion method as well as the E- test method. Results: Categorical agreement between the methods was 100% for isoniazid, rifampin, streptomycin, and ethambutol. Conclusion: The E- test method appears to be an alternative method to agar proportion for testing the susceptibility of M. tuberculosis isolates to the first- line antituberculous agents. Copyright (C) 2005 S. Karger AG, Basel.