Immobilization of laccase for biotechnology applications


Sanlier S. H. , GIDER S., KOPRULU A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.41, no.4, pp.259-263, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.3109/10731199.2012.731414
  • Title of Journal : ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Page Numbers: pp.259-263
  • Keywords: Alginate, Direct Blue 2, dye decolorization, enzyme immobilization, laccase, DECOLORIZATION, DYES, NANOPARTICLES

Abstract

Laccase played an important role in the decolorization of wide spectrum dyes as a low-cost and environmentally friendly technology. Laccase was immobilized in alginate beads and immobilization conditions were identified. 25 mg/ml laccase enzyme encapsulation efficiencies of using the prepared bead was calculated as approximately 94%. At the end of the 10 days of storage, the free laccase and immobilized laccase retained about 8.08% and 80.83%, respectively. The decolorization of the dye (Direct Blue 2) was around 86% for immobilized enzyme at 45 degrees C. In the study, compared to the free enzyme, high activity, stable, reusable immobilized enzyme preparation was prepared.