An organo-montmorillonite was synthesized to generate a two-level antibacterial agent. The material (Mt-CP-SR) was prepared through the adsorption of sodium lauroyl sarcosinate (SR) on montmorillonite modified with cetylpyridinium (Mt-CP) and its structure was characterized by conducting ATR-FTIR, XRD, and SEM analyses. The results of the ATR-FTIR analyses indicated that SR on the surface could be detected when its amount exceeded the CEC of the Mt. The XRD analyses revealed that the adsorption of CP and SR causes the separation of Mt layers into smaller stacks. The adsorption and desorption study of SR onto and from 0.7 CEC Mt-CP were investigated to determine the amount of SR adsorbed at varying initial SR concentrations and the amount of SR released when diluted with water. Around 140 mg of SR could be loaded on one gram of the Mt intercalated with 170 mg of CP. The results indicated that desorption of SR from the surface is gradual and SR and CP have strong interactions on the montmorillonite (Mt) surface. The antibacterial activity of the material was tested against E. coil, S. aureus, and P. aeruginosa. Additionally, the SR solutions and CP + SR solutions in equimolar ratios were subjected to antibacterial tests for comparison purposes. SR proved to be effective against all three bacteria and the MIC values were found as 75 mg/mL for E. coil, 37.5 mg/mL for S. aureus, and 300 mg/mL for P. aeruginosa. The MBC was 300 mg/mL for E. coil and S. aureus. The solutions of CP + SR mixtures were ineffective against P. aeruginosa, whereas, they were effective against S. aureus. The prepared Mt-CP-SR samples were found to be effective against S. aureus and E. coil. These results indicate that the material could be used in antibacterial liquid soaps, in toothpaste formulations, personal care products, and topical applications against acne, and wounds without any negative contribution to the physico-chemical and detergency properties of the materials.