LC-MS/MS measurement of leukocyte cystine; effect of preanalytic factors.

Canbay E. , Sezer E. , Uçar S. , Çoker M., Sözmen E.

Talanta, vol.209, pp.120558, 2020 (Journal Indexed in SCI Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 209
  • Publication Date: 2020
  • Doi Number: 10.1016/j.talanta.2019.120558
  • Title of Journal : Talanta
  • Page Numbers: pp.120558


Cystinosis is an autosomal recessive disorder characterized by the accumulation of cystine in lysosomes, causing irreversible damage to organs, especially the kidneys. Intracellular leukocyte cystine concentrations are used to diagnose cystinosis and to monitor cysteamine treatment. The aim of this study was to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method without derivatization capable of measuring leukocyte intracellular cystine concentrations.