Magnetic solid phase extraction of erythrosine (E127) in pharmaceutical samples with Fe3O4/C-nanodots hybrid material prior to spectrophotometric analysis


Emiroglu E., YUVALI D., SARP G., YILMAZ E., NARİN İ.

MICROCHEMICAL JOURNAL, vol.170, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 170
  • Publication Date: 2021
  • Doi Number: 10.1016/j.microc.2021.106766
  • Title of Journal : MICROCHEMICAL JOURNAL
  • Keywords: Carbon nanodots, Erythrosine, Magnetic solid phase extraction, Preconcentration, Separation, UV-Vis spectrophotometer, SYNTHETIC COLORANTS, FOOD, ADSORPTION, DYES

Abstract

In this study, a green production method was used to obtain magnetic carbon nanodot hybrid material (Fe3O4@C-nanodots). The new material was used as adsorbent for the magnetic solid phase extraction (MSPE) of Erythrosine (E127) in pharmaceutical samples prior to UV-VIS spectrophotometry analysis. C-nanodots were produced from pasteurized cow milk by using a simple and cheap hydrothermal synthesis method. After production of the C-nanodots, Fe3O4@C-nanodot hybrid material was fabricated with one-step hydrothermal method in green solvent medium. Experimental variables affecting the extraction efficiency of erythrosine such as pH of sample solution, amount of adsorbent, extraction time, eluent type and volume were studied and optimized in details. The characterization studies for the Fe3O4@C-nanodots were carried out by X-ray diffraction spectrometry (XRD), Raman spectrometry (Raman) and Scanning Electron Microscopy (SEM) analysis. Under the optimum experimental conditions, The limit of detection (LOD) was 60 ng.L-1 and the recoveries at three spiked levels were ranged from 94.8% to 97.6% with the relative standard deviation (RSD) less than 5.0%. The results show that the combination MSPE with UV-VIS spectrophotometer provided a simple and rapid method for extraction and determination of Erythrosine.