Development of a Label Free IGE Sensitive Aptasensor Based on Electrochemical Impedance Spectrometry


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Kara P. , Meric B., Ozsoz M.

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, cilt.13, ss.578-581, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 13 Konu: 7
  • Basım Tarihi: 2010
  • Doi Numarası: 10.2174/1386207311004070578
  • Dergi Adı: COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
  • Sayfa Sayıları: ss.578-581

Özet

A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5' Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analyses are based on electron transfer resistance (R(ct)) in the presence of 5mM [Fe(CN)(6)](3-/4-).