The aim of this study is to determine suitable conditions for egg disinfection of gilthead sea bream (Sparus aurata), red porgy (Pagrus pagrus), and common dentex (Dentex dentex) with glutaraldehyde, hydrogen peroxide and iodine. The eggs were disinfected with 200 ppm glutaraldehyde (T= 2, 4, 8, 16 min), 300 ppm hydrogen peroxide and iodine (T= 5, 10, 15, 20 min) at four different contact times. The hatching rate was determined in triplicates for each treatment. Also, bacteria colonies were counted on tryptic soy agar (TSA) and thiosulphate citrate bile salt sucrose agar (TCBS). At the end of the experiment, bacterial load and hatching rate were assessed together. For glutaraldehyde treatment, optimal hatching rates and bacterial colonies were estimated as CT (Disinfectant concentration in mg.l(-1)* exposure time in minute) 800 for sea bream eggs, CT 1600 for red porgy eggs and CT 800-1600 for common dentex eggs. Also, for hydrogen peroxide treatments, they were determined as CT 3000 for sea bream and red porgy and CT 4500 for common dentex. Finally, in iodine treatments, these parameters were measured as CT 4500-6000 for all species' eggs. However, in our study, it is clear that the glutaraldehyde showed the optimum disinfection effect against the microorganisms because the bacteria could be eliminated completely.