EMU Journal of Pharmaceutical Sciences, vol.3, no.3, pp.159-168, 2020 (National Refreed University Journal)
Lactobacillus plantarum is the most commonly used microorganism in industrial food fermentations.
The multidrug resistance, bacteriocin production, various enzyme activities, probiotic properties and the
resistance of the strain against bacteriophages, are important for application in industrial field.
Microorganisms have developed various survival strategies in the evolutionary process. Genomic antiphage role of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR-Cas) is one of the
defense strategies. This is an RNA-based immune system involved in the collaboration of CRISPRrelated (cas) genes with DNA sequences of foreign genetic elements inserted between the repeated
In this study, a rapid diagnosis of L. plantarum was carried out among 59 pickled olives by Real-Time
Polymerase Chain Reaction (PCR) with species-specific probes and primers. Twenty-five isolates were
identified as L. plantarum. The presence of CRISPR-Cas loci of the genomic anti-phage system in these
identified species was investigated using DNA based and bioinformatics methods such as PCR,
sequencing, and molecular software. Specific primer design for L. plantarum CRISPR arrays was
performed. CRISPR loci were detected in 14 L. plantarum strains via classical PCR method. The results
were analyzed using NCBI-Blast, CRISPRFinder databases. In one of the isolates, 6 repeats, and 5
spacers nucleotide arrays were found. CRISPR-related proteins (Dead/Deah box helicases) were
detected in remaining isolates.