High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies


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Sharma A. D. , Brodskiy P. A. , Petersen E. M. , Dagdeviren M. , Ye E., Mallapragada S. K. , ...Daha Fazla

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Basım Tarihi: 2015
  • Doi Numarası: 10.3791/52242
  • Dergi Adı: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS

Özet

Mesenchymal stem cells (MSCs) derived from bone marrow are a powerful cellular resource and have been used in numerous studies as potential candidates to develop strategies for treating a variety of diseases. The purpose of this study was to develop and characterize MSCs as cellular vehicles engineered for delivery of therapeutic factors as part of a neuroprotective strategy for rescuing the damaged or diseased nervous system. In this study we used mouse MSCs that were genetically modified using lentiviral vectors, which encoded brain-derived neurotrophic factor (BDNF) or glial cell-derived neurotrophic factor (GDNF), together with green fluorescent protein (GFP).