Effects of vitamin E and prednisolone on some oxidative stress markers in endotoxemic rabbits

YAZAR E., KONYALIOĞLU S. , ÇÖL R., BİRDANE Y. O. , BAŞ A. L. , Elmas M.

REVUE DE MEDECINE VETERINAIRE, vol.155, no.11, pp.538-542, 2004 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 155 Issue: 11
  • Publication Date: 2004
  • Title of Journal : REVUE DE MEDECINE VETERINAIRE
  • Page Numbers: pp.538-542
  • Keywords: vitamin E, prednisolone, oxidative stress, endotoxemia rabbit, DISSEMINATED INTRAVASCULAR COAGULATION, ESCHERICHIA-COLI ENDOTOXIN, SUPEROXIDE-DISMUTASE, SALMONELLA-TYPHIMURIUM, ANTIOXIDANT STATUS, RATS, INHIBITION, MICE, OVEREXPRESSION, INTERLEUKIN-1

Abstract

Effects of prednisolone (PR) and vitamin E (VE) on oxidative stress and antioxidant systems were investigated in endotoxemic rabbits. Forty rabbits were used and divided into four equal groups. Group I served as the control group. In group II, lipopolysaccharide (LPS, 100 mug/kg/h) was infused for 6 hours, whereas rabbits of groups III and IV received prior treatments with subcutaneous injection of prednisolone (10 mg/kg) (group III) or with intraperitoneous injections of vitamin E (10 mg/kg) for 4 consecutive days (group IV). Serum, liver, heart and kidney samples were obtained at 8 hours after infusion. Malonedialdehyde (MDA), glutathione (GSH) concentrations and superoxide dismutase (SOD), catalase (CAT) activities were spectrophotometrically determined in tissues plus in serum (for MDA).