Environmental pollution in relation to heavy metals is a worldwide issue. A large number of methods and techniques are utilized to overcome this problem. For this purpose, one of the most widely used techniques is phytoremediation, which is considered as a green technology. The mentioned technique involves the capability of some specific plants to absorb and accumulate heavy metal ions of plant parts. Phytoremediation has received increasing attention starting from the discovery of hyperaccumulator plants, which are able to concentrate high levels of specific metals in their aboveground harvestable biomass. The roots of Nicotiana species with the synthesis and release of nicotine and the precursor - nicotinamide as a significant heavy metal chelator can make tobacco as a potential crop for the aim of phytoremediation. The purpose of this study is the determination of cytoplasmic inheritance role in the heavy metal hyperaccumulation mechanism. Maternal effects for heavy metal (cadmium) hyperaccumulation mechanism were evaluated reciprocal F1 tobacco plant from crosses between the Basma cv. having a hyperaccumulation mechanism and Dubek cv. having not a hyperaccumulation mechanism. When calculated by comparing the progeny means from reciprocal crosses, maternal effects were not significant for any of the crosses. The obtained data indicate that the heavy metal hyperaccumulation mechanism was not related to maternal factors in the tobacco cultivars used in this experiment. The determination of the role of the cytoplasmic heredity in the cadmium accumulation mechanism according to the performance of the crosses is a useful tool from the standpoint of the strategy used in improving of new varieties.