Ultrasound extraction of valonea tannin from the acorn cups of the Quercus species was investigated and compared to the conventional extraction method. Effects of ultrasonic power, time and temperature on extraction were examined. Considering the highest extraction yields and tannin contents of two extraction methods, it was found out that ultrasound extraction result in 17.16% increase in extraction yield and 6.61% in tannin amount. Additionally, apart from the temperature study, the sound effect on extraction yields that occurred during ultrasound extractions was also determined. The results were evaluated statistically by using One-Way ANOVA and Duncan tests with the SPSS 15 statistic program. The results showed that ultrasonic extraction method can be used as an alternative to conventional method for extraction of tannin (valonea) from acorn cups.