The purpose of this study was to examine the relationship and interaction between personality traits and sportspersonship orientations according to gender differences. 510 (235 female, 275 male) professional athletes from various team sports voluntarily participated in this study. The revised version of Eysenck's Personality Questionnaire and Multidimensional Sportspersonship Orientations Scale were used to collect data. The results of this study revealed that female athletes obtained higher scores when compared to males in extraversion-concern and respect for social conventions, respect for rules and officials and commitment towards sport participation subscales. In conclusion, the results of this study indicated that there was a significant relationship between personality subscales and sportspersonship orientation. In addition, personality subscales significantly effected sportspersonship orientations according to gender.