Pyranose oxidase (POx) biosensor based on carbon nanotube (CNT)-modified carbon paste electrode (CNTPE) was developed and characterized using glucose as a substrate. As well as, POx-CNTPEs, unmodified carbon paste electrodes (POx-CPE) were also prepared to examine the effect of CNT addition to the efficiency of the biosensing system and results were given as a comparison of two systems. Apart from analytical characterization, optimization studies and stability tests were carried out. Finally, proposed systems were applied to glucose analysis in wine samples. (C) 2008 Elsevier B.V. All rights reserved.