The present study was carried out to determine the effects of clipping application on seedling and field performance of three tobacco varieties in 2014 and 2015. Akhisar-97, Izmir-Ozbas and Saribaglar-407 tobacco varieties were planted based on randomized complete block design with three replications. In the research, the effects of clipping application on lenght of the seedling, stem diameter, healthy seedlings per square, plant height, number of the leaves, leaf width, leaf lenght and cured leaf yield were evaluated. Average results of two years indicated that clipping application on lenght of seedling, stem diameter, plant height, leaf width, leaf lenght and cured leaf yield were higher than traditional method. Akhisar-97 had statistically high performance than the Izmir-Ozbas and Saribaglar-407 tobacco varieties. The highest lenght of the seedling was obtained in Akhisar-97 (21.0 cm) tobacco cultivar by using clipping application for both years. Cured leaf yield results indicated that Akhisar-97 tobacco variety was the highest yield as 1037 kg ha(-1) at clipping application whereas the lowest yield was recorded as 717 kg ha(-1) in traditional practice in the second year.