Evaluation of three new culture media for the cultivation and isolation of Leishmania parasites

LİMONCU M. E. , ÖZBİLGİN A., BALCIOĞLU İ. C. , Ozbel Y.

JOURNAL OF BASIC MICROBIOLOGY, vol.44, no.3, pp.197-202, 2004 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 3
  • Publication Date: 2004
  • Doi Number: 10.1002/jobm.200310367
  • Title of Journal : JOURNAL OF BASIC MICROBIOLOGY
  • Page Numbers: pp.197-202

Abstract

The aim of this study was to establish novel culture media for Leishmania parasites with a potential of obtaining high amounts of promastigotes with long-term viability, and consisting of ingredients that were available in microbiology or parasitology laboratories. Other features of these media included no requirement for blood, FCS (Fetal calf serum) or erythrocyte lysate, inexpensiveness and easiness in preparation. In addition, aspiration samples obtained from cutaneous leishmaniasis (CL) suspected patients were cultivated in these media.