Fatty acid (FA) values, including C8:0, C10:0, C12:0, C13:0, C14:0, C16:0, C18:0, C18:1t, C18:1c, C18:2, C18:2 CLA, C20:0, C18:3 and C22:0, in sixteen infant formulas were determined through direct transesterification of acyl lipids with sodium methoxide by capillary gas chromatography. Total FA values ranged from 250.25-256.06 mg/g sample and the conjugated linoleic acid (CLA) (cis-9, trans-11 octadecadienoic acid) values were 1.41-2.02 mg/g sample (p < 0.01) in the infant formulas. The total saturated fatty acid, monounsaturated fatty acid and polyunsaturated fatty acid values were also obtained. The C18:3 (linolenic acid (n-3)) values were 0.63-0.88 mg/g sample, whereas the C18:2 (linoleic acid (n-6)) values ranged from 35.64-35.82 mg/g sample. FA standard mix including CLA had linear calibration curves through the origin (R-2 = 0.9999). The precision of the analytical method was (using C13:0, methyl tri-decanoate, as internal standard) within the 95% confidence limits and the mean recoveries determined for individual fatty acids in infant formulas varied from 99.8 to 100%.