Mannose based polymeric nanoparticles for lectin separation

Demir E. F. , Atay N. O. , Koruyucu M. , Kök G. , SALMAN A. Y. , AKGÖL S.

SEPARATION SCIENCE AND TECHNOLOGY, cilt.53, ss.2365-2375, 2018 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 53 Konu: 15
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/01496395.2018.1452943
  • Sayfa Sayıları: ss.2365-2375


The aim of this work is to synthesize the original, new polymeric nanoparticles for concanavalin A (Con A) purification. Nanoparticles were synthesized by surfactant free emulsion polymerization. In the polymerization prosedure, 1-O-(2'-hydroxy-3'-acryloyloxypropyl)-2,3:5,6-di-O-isopropylidene-alpha-D-mannofuranose (Man-OPA) was used as co-monomer and 2-hydroxyethylmethacrylate (HEMA) was used as a monomer. Man-OPA was characterized by Fourier Transform Infrared Spectroscopy (FTIR), nuclear magnetic resonance and elemental analysis techniques. Poly(HEMA-Man-OPA) nanoparticles were characterized by scanning electron microscopy, FTIR and Zeta Sizer. In adsorption-desorption experiments, maximum Con A adsorption capacity of poly(HEMA-Man-OPA) nanoparticles was found 630.6 mg/g nanoparticle (pH 7.5, 1.0 mg/mL). Adsorption-desorption experiments were repeated in four times. According to results, these nanoparticles could be used several times without significant decrease in Con A adsorption capacity.