alpha-Amylase Monitoring by a Novel Amperometric Biosensor Based on Au Electrode: Its Optimization, Characterization, and Application


MENGÜLLÜOĞLU U. , ALTUĞ Ç. , ERTUGRUL H. D. , YILDIZ A., EKICI E. M. , DİNÇKAYA E.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.40, ss.97-102, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 40
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/10731199.2011.597757
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.97-102

Özet

A low-cost and sensitive amperometric biosensor was developed for the determination of alpha-amylase activity. The biosensor was constructed by immobilizing glucose oxidase-gelatin via glutaraldehyde on the Au electrode surface. Measurements were carried out chronoamperometrically at -0.7 V. Several parameters such as glucose oxidase activity, gelatin amount, and glutaraldehyde percentage for cross-linking were optimized. Optimum pH, optimum temperature, repeatability, and storage stabilities of the biosensor were identified. Under the optimum experimental conditions, a linear calibration curve was obtained for alpha-amylase between 0.819 and 13.110 U/ml. Sample analyses were carried out by detecting alpha-amylase activities in baker's yeast samples.