Softeners are of great importance in textile processing. The aim of this treatment is to achieve a soft handle to facilitate the processability and improve wettability. In this study, it was aimed to investigate the effect and washing durability of various softeners on handle properties of cotton and bamboo knitted fabrics. Six types of softeners were applied to the fabrics. Moreover, the washing durability of the softeners after 5, 10 and 20 washing cycles were tested. Handle characteristics such as drapeability, kinetic friction coefficient and circular bending rigidity were measured. The results were statistically evaluated. It was determined that softening treatment does not have a successful performance in bamboo fabrics as it has in cotton fabrics.