The new compound 2-O-beta-D-fructofuranosylglycerol was isolated from the hexane residue. Also 2-O-methyl-alpha-fructofuranoside was isolated for the first time from the fruits of Pyrus amygdaliformis Vill. The chemical structure of isolated carbohydrates was identified using NMR, mass spectroscopic technique and literature data. The minimal inhibitory concentrations (MIC) of P. amygdaliformis Vill. hexane, dichloromethane and butanol extracts of fruits were used for testing microorganisms. Hexane extract showed antimicrobial activity against Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis and Salmonella typhimurium with MIC ranging between 25 and 0.39 mg/mL. The most sensitive strain was found to be K. pneumoniae with MIC value at 0.39 mg/mL in hexane extract.