Radiolabeled D-Penicillamine Magnetic Nanocarriers for Targeted Purposes


OZYUNCU S. Y. , TEKSÖZ S. , İÇHEDEF Ç. , MEDİNE E. İ. , Avci C. B. , GÜNDÜZ C. , ...More

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, vol.16, no.4, pp.4174-4179, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 4
  • Publication Date: 2016
  • Doi Number: 10.1166/jnn.2016.11646
  • Title of Journal : JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
  • Page Numbers: pp.4174-4179
  • Keywords: D-Penicillamine, Magnetic Nanoparticles, Radiolabeling, MCF7 Cell Line, In Vitro, IRON-OXIDE NANOPARTICLES, IN-VITRO, BIOMOLECULES, TC-99M, PARTICLES, BEHAVIOR, CARRIERS, AGENTS

Abstract

The aim of this study is to synthesize D-Penicillamine (D-PA) conjugated magnetic nanocarriers for targeted purposes. Magnetic nanoparticles were prepared by partial reduction method and surface modification was done with an amino silane coupling agent's (structural properties), AEAPS, the particles were characterized by Scanning Electron Microscope (SEM), X-ray Diffraction (XRD). After that D-PA was linked with the magnetic nanoparticles (MNPs) and has been radiolabeled with [Tc-99m(CO)(3)](+) core. Quality controls of [Tc-99m(CO)(3)-MNP-D-PA] were established by Cd(Te) detector. The radiolabeling efficiency of magnetic nanoparticles ([Tc-99m(CO)(3)-MNP-D-PA]) was about 97.05% with good in vitro stability during the 24 hour period. As a parallel study, radiolabeled D-PA complex ([Tc-99m(CO)(3)-D-PA]) was prepared with a radiolabeling yield of 97.93%. At the end, biologic activities of binding complexes were investigated on MCF7 human breast cancer cells. Our results show that, radiolabeled magnetic nanoparticles with core[Tc-99m(CO)(3)](+) ([(99)mTc(CO)(3)-MNP-D-PA]) showed the highest uptake on MCF7 cells which were applied magnetic field in the wells. In that case, result of this study emphasizes that radiolabeled magnetic nanoparticles with core [Tc-99m(CO)(3)](+) would support new occurrences of new agents.