The present study was designed to identify and compare the in vivo wound healing capacity of a bark extract from Pinus brutia and Pycnogenol(R) in an incision wound model in rats. O/W cream formulations were prepared incorporating 2% Pycnogenol(R) and P brutia bark extract. The rats were divided into three groups (n = 8). Subsequently placebo and test formulations were applied to animals once a day from day "0" until the 9th day. Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were studied in addition to histopathological examinations. Treatment with P brutia extract containing cream inhibited lipid peroxidation by a 35 % decrease in MDA and 46.8 % increase in SOD activity, whereas 19.3 % decrease in MDA and 34.7 % increase in SOD activity were attained with Pynogenol(R) compared to control. The histological data revealed a better performance of P brutia extract enriched formulation in terms of degeneration of hair roots, increased vascularization and a decrease in necrotic area. Consequently, a high wound healing activity was observed in animals treated with P brutia extract significantly accelerating the wound healing process.