Enzymatic synthesis of I-125/131 labeled 8-hydroxyquinoline glucuronide and in vitro/in vivo evaluation of biological influence


YESILAGAC R., ÜNAK P. , MEDİNE E. İ. , İÇHEDEF Ç. , Ertay T., Muftuler F. Z. B.

APPLIED RADIATION AND ISOTOPES, cilt.69, ss.299-307, 2011 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 69 Konu: 2
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.apradiso.2010.10.027
  • Dergi Adı: APPLIED RADIATION AND ISOTOPES
  • Sayfa Sayısı: ss.299-307

Özet

8-Hydroxyquinoline (8-OHQ) is a long-known molecule which due to its metal-complexation ability is frequently used for analysis. It is also called oxine. Oxine and derivatives have been investigated to process antitumor and antimicrobial activities. 8-Hydroxyquinolyl-glucuronide (8-OHQ-Glu) was enzymatically synthesized using microsome preparates separated from Hutu-80 cells, labeled with I-125 to perform a radionuclide labeled prodrug and investigated of its biological affinities on Hutu-80 (human duodenum intestinal adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), Detroit 562 (human pharynx adenocarcinoma) cells and ACBRI 519 (primary human small intestine epithelial cells) in this work. UDP-glucuronyl transferase (UDPGT) rich microsome preparates, which are used for glucuronidation in enzymatic synthesis, were extracted from Hutu-80 cells.