Grain protein content of wheat is important for bread making and pasta quality. Wild tetraploid wheat (Triticum turgidum L. var. dicoccoides) has some genes for high protein content as a promising source. Three winter wheat cultivars of Colorado (Halt, Yuma, Akron) and one experimental line (CO99508) and one accession of T. dicoccoides were crossed to obtain backcrosses. The objective of this study was to determine higher protein content, SDS-sedimentation and hardiness values in backcrosses T. aestivum x T. dicoccoides.