The aim of this study is to determine the estimation of hemoglobin concentration and oxygen saturation of tissue by non-invasively functional laser imaging for early skin cancer diagnosis. The early diagnosis of melanoma is a key factor that remarkably reduces the mortality rate. Diffuse reflectance spectroscopy is a very useful device for diagnosis and treatment purposes under in-vivo conditions. At this point, the aforementioned device, which takes into account the scattering of tissue, is to determine the concentration of chromophores (or optical absorbers) due to attenuated light strikes to the superficial layer of tissue. Laser-type light based imaging techniques in medical diagnosis substantially produce good results. Consequently, the aim of this study is to estimate HbO(2) (%) and Hb (%) concentrations by use of a single wavelength (680 nm).