Scale-up and kinetic modeling for bioethanol production

İMAMOĞLU E. , Sukan F. V.

BIORESOURCE TECHNOLOGY, vol.144, pp.311-320, 2013 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 144
  • Publication Date: 2013
  • Doi Number: 10.1016/j.biortech.2013.06.118
  • Page Numbers: pp.311-320
  • Keywords: Bioethanol production, Scale-up, Shaken flask, Stirred-tank bioreactors, Kinetic modeling, ETHANOLOGENIC ESCHERICHIA-COLI, BATCH FERMENTATION KINETICS, ZYMOMONAS-MOBILIS, ACID PRODUCTION, PERFORMANCE, BIOMASS, FLASKS, FUELS, KO11


Bioethanol was produced from acidic hydrolysate of rice hulls using recombinant Escherichia coli KO11. Two different issues (scale-up and kinetic modeling) were evaluated simultaneously and concomitantly for bioethanol production. During the step-wise scale-up process from 100 mL shaken flask to 10 L stirred-tank bioreactor, the constant Reynolds number and the constant impeller tip speed were evaluated as scale-up methodologies under laboratory conditions. It was determined that the volumetric bioethanol productivity was 88% higher in 10 L bioreactor in comparison to the value of 0.21 g L-1 h(-1) in shaken flask. The modified Monod and Luedeking-Piret models provided an accurate approach for the modeling of the experimental data. Ethanol concentration reached the maximum level of 29.03 g/L, which was 5% higher than the value of model prediction in 10 L bioreactor. The findings of this research could contribute to the industrial scale productions especially from lignocellulosic raw materials. (C) 2013 Elsevier Ltd. All rights reserved.