Synthesis, characterization and biodistribution of Tc-99m-Bioquin-HMPAO (Tc-99m-BH) as a novel brain imaging agent


KILCAR A. , MUFTULER F. Z. B. , Enginar H., TEKIN V., MEDİNE E. İ. , ÜNAK P.

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, vol.302, no.1, pp.563-573, 2014 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 302 Issue: 1
  • Publication Date: 2014
  • Doi Number: 10.1007/s10967-014-3175-1
  • Title of Journal : JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Page Numbers: pp.563-573
  • Keywords: Brain imaging, HMPAO, Bioquin, Tc-99m, ALZHEIMERS-DISEASE, AMYLOID PLAQUES, COMPLEXES, NANOPARTICLES, COGNITION, PROGRESS, BINDING, UTILITY, PBT2, MICE

Abstract

Recently, the development of novel brain imaging agents has aroused much interest thanks to limited number of brain cancer or diseases diagnosis agents. It is aimed to synthesize a novel brain imaging agent including a promise for further studies on AD diagnosis potential and investigate its bioaffinity with biodistribution studies on healthy Balb/c mice. A novel radiolabeled agent was synthesized and characterized. Quality control of Tc-99m-BH was performed utilizing solvent extraction and chromatographic (Radio TLC and Radio HPLC) methods. Bioaffinity of the Tc-99m-BH was investigated on male Balb/c mice at various time points (5, 30, 60, 120 min post-injection). Paper electrophoresis showed that Tc-99m-BH has a neutral structure. Radiochemical purity of Tc-99m-BH was over 95 % with appropriate stability for imaging period. Selected brain regions have uptakes over 4 % ID/g following intravenous injection. Hippocampus has uptake approximately 10 % ID/g. Tc-99m-BH has shown brain uptake, so it may prove to be valuable for brain imaging as a novel technetium-labeled agent. Further investigations with AD animal model are our on going effort to show that this agent has AD diagnosis potential.