Methacryloylamidohistidine in Affinity Ligands for Immobilized Metal-ion Affinity Chromatography of Ferritin


Uygun D. A. , Ozturk N. , AKGÖL S. , DENİZLİ A.

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, vol.16, no.1, pp.173-179, 2011 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 1
  • Publication Date: 2011
  • Doi Number: 10.1007/s12257-009-0162-4
  • Title of Journal : BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
  • Page Numbers: pp.173-179

Abstract

A new metal-chelate adsorbent utilizing 2-methacryloylamidohistidine (MAH) was prepared as a metal-chelating ligand. MAH was synthesized using methacryloly chloride and histidine. Monosize nanospheres with an average diameter of 450 nm were produced by emulsion polymerization of 2-hydroxyetylmethacrylate (HEMA) and MAR Then, Fe(3+) ions were chelated directly onto the monosize nanospheres. Mon-poly(HEMA-MAH) nanospheres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and elemental analysis. Fe(3+) chelated monosize nanospheres were used in ferritin adsorption from an aqueous solution. The maximum ferritin adsorption capacity of Fe(3+)-chelated mon-poly(HEMA-MAH) nanospheres was 202 mg/g at pH 4.0 in acetate buffer. The non-specific ferritin adsorption on the monpoly(HEMA-MAH) nanospheres was 20 mg/g. The adsorption behavior of ferritin could be modeled using both Langmuir and Freundlich isotherms. The adsorption capacity decreased with increasing ionic strength of the binding buffer. High desorption ratios (> 95% of the adsorbed ferritin) were achieved with 1.0 M NaCl at pH 7.0. Ferritin could be repeatedly adsorbed and desorbed with the Fe(3+)-chelated mon-poly(HEMA-MAH) nanospheres without significant loss of adsorption capacity.