Lipase biosensor for tributyrin and pesticide detection


Kartal F. , Kilinc A. , Timur S.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, cilt.87, ss.715-722, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 87
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1080/03067310701327741
  • Dergi Adı: INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
  • Sayfa Sayıları: ss.715-722

Özet

Potentiometric biosensors based on Candida rugosa lipase was described for the detection of organophosphorus pesticide; methyl-parathion and tributyrin. Lipase was immobilized on the glass electrode by means of a gelatin membrane, which is then cross-linked with glutaraldehyde. The principle of the biosensor is based on the measurement of pH variation which was recorded in millivolts due to the enzymatic hydrolysis of tributyrin to butyric acid. For the inhibitor detection, biosensor responses were measured after pesticide treatment, which caused a drop in enzyme activity because of the irreversible inhibition. Reactivation conditions of the reused enzyme electrodes were also investigated by pyridine-2-aldoxime methiodide (2-PAM). The limit of detection for tributyrin was estimated as 93 mu M for lipase sensor within the linear range of 65-455 mu M.