Background: The purpose of this study is to evaluate the nephrotoxicity of sevoflurane inhalation anesthesia applied during carbon dioxide pneumoperitoneum ( CO2-PNP) which is conducted for a laparoscopic surgery treatment. Materials and Methods: 14 New Zealand white rabbits were used in this study. Initially, anesthesia was induced using 3.7% concentrated sevoflurane + 50% O-2/N2O. Then, a tracheotomy was performed, and the rabbits were mechanically ventilated. The first group ( n = 7) was subjected to CO2-PNP for 90 min with a constant intraabdominal pressure of 12 mm Hg, the second ( control group) ( n = 7) was exempted. The serum inorganic fluoride ( IF-) concentration was measured. The rabbits were sacrificed after 72 h, and one kidney each was immediately extracted for histopathological examination. Results: Serum IF- concentrations were not different in both groups. Histopathologically, mild renal damage was found in one rabbit in each group. Conclusion: CO2-PNP did not have any additional effect on the nephrotoxicity of sevoflurane.