PREPARATION AND CHARACTERIZATION OF CHITOSAN-ENTRAPPED MICROSOMAL UDP-GLUCURONYL TRANSFERASE


ZIHNIOGLU F. , TELEFONCU A.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY, cilt.23, ss.545-552, 1995 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 23 Konu: 4
  • Basım Tarihi: 1995
  • Doi Numarası: 10.3109/10731199509117969
  • Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY
  • Sayfa Sayıları: ss.545-552

Özet

The hepatic microsomal UDP-Glucuronyl transferase which catalyze the glucuronidation of drugs, pesticides, carcinogens and other xenobiotics, was immobilized by entrappment in chitosan. Chemical and physical characterization were made by using l-naphthol as substrate. Thermal, storage and operational stabilities of the immobilized enzyme was also searched and found to be better in comparison with the free enzyme. In conclusion, chitosan gel beads appear to have good characteristics for use as UDPGT immobilization support suitable for large scale use and offer the prospect that immobilized UDPGT may become an important form of catalyst in medicine.