Biodegradation of Direct Blue 15 by Free and Immobilized Trametes versicolor

Pazarlioglu N. K. , AKKAYA A. , Akdogan H. A. , Gungor B.

WATER ENVIRONMENT RESEARCH, cilt.82, ss.579-585, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 82 Konu: 7
  • Basım Tarihi: 2010
  • Doi Numarası: 10.2175/106143009x12529484815999
  • Sayfa Sayıları: ss.579-585


To investigate biodegradability by Trametes versicolor, five structurally different direct azo-dyes-Direct Black 38, Direct Blue 15 (DB 15), Direct Orange 26, Direct Green 6, and Direct Yellow 12-were studied. The DB 15 was determined as the best biodegradable dye by this white-rot fungus. Laccase and manganese peroxidase activities were monitored with the biodegradation process; it was observed that laccase played an important role in the dye degradation, while manganese peroxidase activity could not be detected. Possible degradation products also were examined by gas chromatography-mass spectrometry, but no metabolite was detected after the degradation and/or decolorization process. To enhance performance of the fungi during the degradation, Trametes versicolor cells were immobilized in alginate beads. Then, DB 15 decolorization by immobilized Trametes versicolor was studied in a small-scale packed-bed reactor. The color removal efficiency in repeated batches was found to be 98 and 93% for 50 mg/L DB 15. Water Environ. Res., 82, 579 (2010).