Preparation and evaluation of effect on Escherichia coli and Staphylococcus aureus of radiolabeled ampicillin-loaded graphene oxide nanoflakes

Yurt F. , Ersoz O. A. , Harputlu E., Ocakoglu K.

CHEMICAL BIOLOGY & DRUG DESIGN, cilt.91, ss.1094-1100, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 91 Konu: 6
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1111/cbdd.13171
  • Sayfa Sayıları: ss.1094-1100


Ampicillin is a one of effective antibiotics against Gram-positive and Gram-negative bacteria. This study aimed to label ampicillin-loaded graphene oxide nanoflake (AMP-GO) with Tc-99m and evaluate of its in vitro binding to Staphylococcus aureus and Escherichia coli. Firstly, ampicillin was loaded into graphene oxide nanoflake prepared. AMP-GO was characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) techniques, and the amount of loaded ampicillin onto GO was determined by UV-Vis absorption spectroscopy. AMP and AMP-GO were labeled with Tc-99m using stannous chloride reducing agent. Labeling efficiency of Tc-99m-AMP-GO was found to be 97.66 +/- 2.06%. Tc-99m-AMP-GO has higher binding efficiencies to both S. aureus and E.coli than Tc-99m-AMP. Tc-99m-AMP-GO could be promising candidate as agent infection nuclear imaging. Furthermore, in vivo studies of Tc-99m-AMP-GO with infected rats are planned to be performed.