Among the economically viable and ecologically acceptable solutions for the safe disposal of waste glycerol coming from biodiesel production, the biotechnological conversion of glycerol into a very high-value-added bioplastic raw material, namely 1,3 propanediol (1,3-PDO) seems to be very promising. This process is easy and environmentally friendly since it produces no toxic wastes. The results indicate that glycerol by-product is as amenable for 1,3-PDO production as pure glycerol. The immobilization process has important advantages such as smaller reactor volumes, shorter fermentation times, and higher yields. This work involves the study of 1,3-PDO production with industrial raw glycerol via immobilized culture of Clostridium beijerinckii B-593 on glass beads and glass rushing rings with industrial glycerol. Thereby, maximum volumetric productivity of 7 g L-1 h(-1) corresponding to a product concentration of 13 g L-1 was achieved at 2 hours of hydraulic retention time (HRT) on glass beads. It was demonstrated that continuous production of 1,3-PDO from waste glycerol is feasible and the immobilized bioreactor systems outcompeted the conventional suspended culture bioreactors in terms of both productivity and operational stability.