Electrochemical deposition of polypeptides: bio-based covering materials for surface design

Akbulut H., YAVUZ M., GULER E. , Demirkol D. , ENDO T., YAMADA S., ...More

POLYMER CHEMISTRY, vol.5, no.12, pp.3929-3936, 2014 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 5 Issue: 12
  • Publication Date: 2014
  • Doi Number: 10.1039/c4py00079j
  • Title of Journal : POLYMER CHEMISTRY
  • Page Numbers: pp.3929-3936


A simple and efficient approach for the electrochemical deposition of polypeptides as bio-based covering materials for surface design is described. The method involves N-carboxyanhydride (NCA) ring-opening polymerization from its precursor to form a thiophene-functionalized polypeptide macromonomer (T-Pala), followed by electropolymerization. The obtained conducting polymer, namely polythiophene-g- polyalanine (PT-Pala), was characterized and utilized as a matrix for biomolecule attachment. The biosensing applicability of PT-Pala was also investigated by using glucose oxidase (GOx) as a model enzyme to detect glucose. The designed biosensor showed a very good linearity for 0.01-1.0 mM glucose. Finally, the antimicrobial activities of newly synthesized T-Pala and PT-Pala were also evaluated by using the disc diffusion method.