Development of Fluorescent Array Based on Sol-Gel/Chitosan Encapsulated Acetylcholinesterase and pH Sensitive Oxazol-5-one Derivative


ÖZTÜRK G., FELLER K., DORNBUSCH K., TİMUR S. , Alp S., Ergun Y.

JOURNAL OF FLUORESCENCE, cilt.21, ss.161-167, 2011 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 21 Konu: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s10895-010-0700-7
  • Dergi Adı: JOURNAL OF FLUORESCENCE
  • Sayfa Sayıları: ss.161-167

Özet

A highly sensitive fluorescent enzyme array for quantitative acetylcholine detection is developed. The enzyme array has been constructed by spotting of pH sensitive fluorophore 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacycloopentadecyl)benzylidene]oxazol-5-one and acetylcholinesterase doped in tetraethoxysilane / chitosan matrix via a microarrayer. The constructed tetraethoxysilane / chitosan network provided a microenvironment in which the enzyme molecule was active biologically. The optimal operational conditions for the array developed were investigated. The response of the developed biosensor array to acetylcholine was highly reproducible (RSD = 3.27%, n = 6). A good linearity was observed for acetylcholine in the concentrations up to 1 x 10(-8) M, with a detection limit of 0.27 x 10(-8) M.