Thrombin immobilization to enzymatic modified PET and PAN fabrics and their applications


AKKAYA A. , PAZARLIOGLU N. K.

FIBERS AND POLYMERS, cilt.13, ss.985-993, 2012 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 13 Konu: 8
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s12221-012-0985-z
  • Dergi Adı: FIBERS AND POLYMERS
  • Sayfa Sayıları: ss.985-993

Özet

Enzymatic modification of synthetic materials has immense potential both of the functionalization of polymeric materials, such as poly(acrylonitrile) or polyesters, and the production of polymers for special applications, such as medical devices and enzyme immobilization. In this study, poly(ethyleneterephtalate) and poly(acrylonitrile) fabrics were modified with commercial laccase and nitrilase, respectively. Contact angles of enzymatic modified and unmodified fabrics were measured and it was found contact angles of enzymatic modified fabrics were less than those of unmodified fabrics. Attenuated-Total-Reflection-Fourier-Transform infrared spectroscopy showed that carboxylic acid groups occurred on fabrics after enzymatic modifications. Surfaces of modified and unmodified fabrics were investigated using scanning electron microscopy. Surfaces of unmodified fabrics were smooth but surfaces of modified fabrics were rugged and cracked. Thrombin was immobilized in modified fabrics by using 1-Ethyl-3-(3-dimetylaminopropyl)-carbodiimide. Optimization studies were also performed for the immobilization of thrombin. After prepared material was tested to stop bleeding in vitro conditions and it was found that thrombin immobilized poly(ethyleneterephtalate) and poly(acrylonitrile) fabrics had a reduced recalcification time to 51 % and 89 %, respectively. Thrombin immobilized poly(ethyleneterephtalate) fabric was also tested in in vivo conditions by using Cavia porcellus and it was observed that this material caused bleeding to stop at a ratio of 24.6 %. The results were statistically significant.