The Protective Effect of Granulocyte Colony-Stimulating Factor on Endometrium and Ovary in a Rat Model of Diabetes Mellitus

Pala H. G. , PALA E. E. , Ulkumen B. A. , AKTUĞ H. , YAVAŞOĞLU A. , KORKMAZ H. A. , ...More

GYNECOLOGIC AND OBSTETRIC INVESTIGATION, vol.78, no.2, pp.94-100, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 78 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1159/000363239
  • Page Numbers: pp.94-100
  • Keywords: Granulocyte colony-stimulating factor, Diabetes mellitus, Ovarian injury, Endometrial dysfunction, Antimullerian hormone, Oxidative stress, GLYCATION END-PRODUCTS, G-CSF, HORMONE, WOMEN, EXPRESSION, MENOPAUSE, ISCHEMIA, DEATH, CELLS, AGE


Aims: To evaluate the effect of granulocyte colony-stimulating factor (G-CSF) on the endometrium and ovaries in an experimental diabetes mellitus (DM) rat model. Methods: A total of 18 female Sprague-Dawley albino mature rats (8 weeks, 200-220 g) were used in this study. Diabetes was induced by intra peritoneal (i.p.) injection of streptozocin randomly in 12 rats. No drug was administered to the remainder of the rats (control group, group 1, n = 6). The other 12 rats were randomly divided into 2 groups; 1 ml/kg i.p. saline was given as vehicle to group 2 (diabetic nontreated control group, n = 6) and 100 mu g/kg/day of i.p. G-CSF was given to group 3 (G-CSF-treated group, n = 6) for 4 weeks. After 4 weeks, blood samples were collected and hysterectomy with bilateral oophorectomy was performed for histopathological examination. Results: The mean endometrial gland degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Ovarian follicle degeneration, stromal degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Plasma TGF-beta and malondialdehyde levels were significantly lower in groups 1 and 3 compared with group 2. Antimullerian hormone levels were significantly lower in group 2 compared with groups 1 and 3. Conclusion: Glucose toxicity occurred severely in the ovaries and endometrium of the DM rats. After G-CSF treatment, ovarian and endometrial injury and fibrosis scores decreased significantly. The effects of G-CSF in rat models give hope to improved treatment of human DM complications such as premature ovarian failure and endometrial dysfunction. (C) 2014 S. Karger AG, Basel